VEN123L Video 9.1 - Limits of Detection and Quantification
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VEN123L Video 9.1 - Limits of Detection and Quantification
What's the lowest concentration that a method or instrument can detect, and what's the lowest it can measure? What's the difference? How do we determine the limits of detection and quantification?Copyright Thi NguyenDo not distribute without [email protected]
Jan 23, 2021
Detection Limit Analysis
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Example: A new electrochemical method relates concentration to current. The slope of the calibrationcurve is 0.438 nA/µM. Replicate analysis of a low concentration solution gave the following readings:2.39 nA, 2.45 nA, 2.54 nA, 2.76 nA and 2.58 nA.7. (0.5 point) What is the detection limit of this method?8. (0.5 point) What is the lowest concentration that could be reliably quantified?9. (1 points) Why do you think the "bar" is set higher for quantification than detection?
Example: A new electrochemical method relates concentration to current. The slope of the calibrationcurve is 0.438 nA/µM. Replicate analysis of a low concentration solution gave the following readings:2.39 nA, 2.45 nA, 2.54 nA, 2.76 nA and 2.58 nA.7. (0.5 point) What is the detection limit of this method?8. (0.5 point) What is the lowest concentration that could be reliably quantified?9. (1 points) Why do you think the "bar" is set higher for quantification than detection?
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