Stimulated Emission Depletion (STED) microscopy is a super-resolution imaging technique that overcomes the diffraction limit of conventional optical microscopy. It enables the visualization of ...

The 2014 Nobel Prize in Chemistry was awarded for the development of super-resolved fluorescence microscopy, including STED (Stimulated Emission Depletion) microscopy. This method can be used to ...

For a long time, electron microscopy was considered the only way to observe the nanoscale, owing to the diffraction limit of focused light. Super-resolved fluorescence microscopy revolutionized this ...

Super-resolution microscopy enables the observation of cells at unprecedented detail but usually entails high light exposure and slow imaging. Thus, often only a few manually selected regions are ...

Nanoscopy describes the ability to see beyond the generally accepted optical limit of 200–300 nm. Stimulated emission depletion (STED) microscopy, developed by Stefan W. Hell and Jan Wichmann in 1994, ...